UV-VIS Spectrophotometers

JP Selecta spectrophotometers measure absorbance and transmittance of liquid and solid samples in the ultraviolet and visible ranges through quantitative application of the Beer-Lambert law, an essential analytical technique for quantification of proteins, nucleic acids, metal ions, pharmaceutical active ingredients and dyes. For complementary analysis of food and water samples, see our food analysis equipment.

The operating principle relies on selective attenuation of a monochromatic beam passing through the sample, recording transmitted versus incident radiation to calculate absorbance. Equipment performance characterization (wavelength accuracy, photometric accuracy, noise and stray light) follows the ASTM E275 guidance on describing and measuring UV-VIS spectrophotometer performance, complemented by ASTM E925 on calibration monitoring.

The range includes single-beam spectrophotometers in the visible range (320-1100 nm) for routine analysis, double-beam UV-VIS models (190-1100 nm) for demanding applications with automatic drift correction, and diode array models for rapid spectral scanning. Combine with viscometers for complete analytical characterization of liquid formulations.

Applications cover protein quantification by Bradford and BCA methods, nucleic acid purity analysis by A260/A280 ratio, drug determination per USP <857> on UV-VIS spectroscopy and USP <1857> on theory and practice, chromogenic water analysis and determination of molar extinction coefficients. For spectroscopic analysis terminology, see the IUPAC Compendium of Terminology in Analytical Chemistry.

Regarding calibration and traceability, JP Selecta spectrophotometers support wavelength accuracy verification using NIST SRM 2034 holmium oxide reference material (240-640 nm) and photometric verification using certified neutral density filters. The NIST traceability in molecular spectrophotometry program describes the calibration procedure under ISO/IEC 17025.

Manufactured in Abrera under triple certification ISO 9001:2015, ISO 14001:2015 and ISO 13485:2018, JP Selecta spectrophotometers include kinetic, quantitative and spectral scan software, standard cuvettes and official technical service with international traceable calibration. Contact an expert to select the right model for your spectral range, bandwidth and sample throughput.